Basic Local Alignment Search Tool (BLAST) - is a tool for matching sequences of genetic information. BLAST performs fast database searching combined with rigorous statistics for judging the significance of matches. Five BLAST programs search many different combinations of query and database sequences. The BLAST algorithm is described in S.F. Altschul, W. Gish, W. Miller, E.W. Myers, and D.J. Lipman, J. Mol. Biol. 215, 403-10 (1990). BLAST binaries, licensing information, documentation and data are distributed by the National Center for Biotechnology Information http://www.ncbi.nlm.nih.gov/. A distribution of BLAST 1.4.9 optimized for Sun UltraSPARC is included here, along with a set of binaries. Edit and run the BLAST.run script to rebuild the binaries.